Publications

Selected Publications

* co-first authors; †co-senior authors

  • Zhan H, Bhattacharya S, Cai H, Iglesias PA, Huang CH†, & Devreotes PN†. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. Developmental Cell. 2020; 54(5), 608–623.e5. [pdf] (Cover Article)
  • Yang JM, Bhattacharya S, West-Foyle H, Hung CH, Wu TC, Iglesias PA & Huang CH. “Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation.” Nat Commun. 2018; 9(1), 4673. PMID: 30405112. [pdf]
  • Tang M, Wang M, Shi C, Iglesias PA and Devreotes PN, Huang CH. “Evolutionarily conserved coupling of adaptive and excitable networks mediates eukaryotic chemotaxis.” Nat Commun. 2014; 5: 5175. [pdf]
  • Huang CH, Tang M, Shi C, Iglesias PA and Devreotes PN. “An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration.” Nat Cell Biol. 2013; 15(11): 1307-1316. [pdf]
  • Huang CH, Mandelker D, Schmidt-Kittler O, Samuels Y, Velculescu VE, Kinzler KW, Vogelstein B, Gabelli SB, Amzel LM. “The structure of a human p110α/p85α complex elucidates the effects of oncogenic PI3Kα mutations.” Science. 2007; 318(5857): 1744-8. [pdf]

Complete List of Publications

  • Wu JW*, Yang JM*†, Chen CC, Au G, Wang S, Chern GW, Huang CH†. Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding. iScience (in press).
  • Chen Q*, Xu Y*, Wu JW, Yang JM†, Huang CH†. Decoding Molecular Network Dynamics in Cells: Advances in Multiplexed Live Imaging of Fluorescent Biosensors. Biosensors (Basel). 2025 Sep 17;15(9). PMID: 41002353.
  • Zhan H†, Pal DS, Borleis J, Deng Y, Long Y, Janetopoulos C†, Huang CH†, Devreotes PN†. Self-organizing glycolytic waves tune cellular metabolic states and fuel cancer progression. Nat Commun. 2025 Jul 1;16(1):5563. PMID: 40593532.
  • Wu JW, Yang JM†, Wang S, Chen Y, Huang CH†. Cell barcoding with tandem fluorescent proteins enables high-throughput signaling dynamics analysis. bioRxiv. 2025 Jun 20. doi: 10.1101/2025.06.17.660155. PMID: 40667186.
  • Chen CC, Wang S, Yang JM†, Huang CH†. Targeting Signaling Excitability in Cervical and Pancreatic Cancer Cells Through Combined Inhibition of FAK and PI3K. Int J Mol Sci. 2025 Mar 26;26(7). PMID: 40243705.
  • Wang S*, Chi WY*, Au G, Huang CC, Yang JM†, Huang CH†. Reconstructing Signaling Networks Using Biosensor Barcoding. Methods Mol Biol. 2024;2800:189-202. PMID: 38709485.
  • Wu JW*, Yang JM*†, Chen CC, Au G, Wang S, Chern GW, Huang CH†. Calibration of FRET-based biosensors using multiplexed biosensor barcoding. bioRxiv. 2024. p. 2024.09.06.610346. [pdf]
  • Huang CH†, Albeck J†, Devreotes P†. Editorial: Self-organizing and excitable signaling networks in cell biology. Front Cell Dev Biol. 2024;12:1430911. [pdf]
  • Wang S*, Chi WY*, Au G, Huang CC, Yang JM†, Huang CH†. Reconstructing signaling networks using biosensor barcoding. Methods Mol Biol. 2024;2800:189–202. [pdf]
  • Wang S, Chen CC, Hu MH, Cheng M, Tu HF, Tsai YC, Yang JM, Wu TC, Huang CH, Hung CF. Arginine-linked HPV-associated E7 displaying bacteria-derived outer membrane vesicles as a potent antigen-specific cancer vaccine. J Transl Med. 2024;22(1):378. [pdf]
  • Zhan H†, Pal DS, Borleis J, Janetopoulos C†, Huang CH†, Devreotes PN†. Self-organizing glycolytic waves fuel cell migration and cancer progression bioRxiv. 2024 p. 2024.01.28.577603. [pdf]
  • Wang S, Cheng M, Chen CC, Chang CY, Tsai YC, Yang JM, Wu TC, Huang CH†, Hung CF†. Salmonella immunotherapy engineered with highly efficient tumor antigen coating establishes antigen-specific CD8+ T cell immunity and increases in antitumor efficacy with type I interferon combination therapy. OncoImmunology. 2023; 13(1):2298444. [pdf]
  • Chen CC, Wang S, Yang JM†, Huang CH†. Targeting Ras signaling excitability in cancer cells through combined inhibition of FAK and PI3K. bioRxiv. 2023. p. 2023.06.12.544386. [pdf]
  • Chi WY, Au G, Liang J, Chen CC, Huang CH†, Yang JM†. Imaging and analysis for simultaneous tracking of fluorescent biosensors in barcoded cells. STAR Protocols. 2022; 3(3), 101611. [pdf]
  • Yang JM†, Chi WY, Liang J, Takayanagi S, Iglesias P, Huang CH†. Deciphering cell signaling networks with massively multiplexed biosensor barcoding. Cell. 2021; 184(25), 6193–6206. PMID: 34838160. [pdf] (Cover Article)
  • Peng S, Ferrall L, Gaillard S, Wang C, Chi WY, Huang CH, Roden RBS, Wu TC, Chang YN, Hung CF. Development of DNA Vaccine Targeting E6 and E7 Proteins of Human Papillomavirus 16 (HPV16) and HPV18 for Immunotherapy in Combination with Recombinant Vaccinia Boost and PD-1 Antibody. MBio. 2021;12(1):e03224-20. PMID: 33468698. [pdf]
  • Zhan H, Bhattacharya S, Cai H, Iglesias PA, Huang CH†, & Devreotes PN†. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. Developmental Cell. 2020; 54(5), 608–623.e5. [pdf] (Cover Article)
  • Yang JM†, Chi WY, Liang J, Iglesias P, Huang CH†. Highly multiplexed imaging of biosensors in live cells. bioRxiv. 2020. p. 2020.12.11.419655. [pdf]
  • Yang JM, Bhattacharya S, West-Foyle H, Hung CH, Wu TC, Iglesias PA & Huang CH. “Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation.” Nat Commun. 2018; 9(1), 4673. PMID: 30405112. [pdf]
  • Yu Y, Suryo Rahmanto Y, Lee MH, Wu PH, Phillip JM, Huang CH, Vitolo MI, Gaillard S, Martin SS, Wirtz D, Shih IM, Wang TL. “Inhibition of ovarian tumor cell invasiveness by targeting SYK in the tyrosine kinase signaling pathway.” Oncogene. 2018; doi: 10.1038/s41388-018-0241-0. PMID: 29643476. [pdf]
  • Schiapparelli P, Guerrero-Cazares H, Magaña-Maldonado R, Hamilla SM, Ganaha S, Fernandes EGL, Huang CH, Aranda-Espinoza H, Devreotes P and Quinones-Hinojosa A. “NKCC1 Regulates Migration Ability of Glioblastoma Cells by Modulation of Actin Dynamics and Interacting with Cofilin.” EBioMedicine. 2017; 21: 94-103. PMID: 28679472. [pdf]
  • Hoeller O, Toettcher JE, Cai H, Sun Y, Huang CH, Freyre M, Zhao M, Devreotes PN and Weiner OD. “Gβ tunes coupling between actin oscillators for cell polarity and directional migration.” PLoS Biol. 2016; 14(2): e1002381. [pdf]
  • Huang CH† and Devreotes PN†. “Reaction diffusion waves of phosphoinositides in the membrane.” ASBMB Today. 2015; 14(8): 16-17. [pdf]
  • Huang CH† and Iglesias PA† . “Cell memory and adaptation in chemotaxis.” Proc Natl Acad Sci U S A. 2014; 111(43): 15287-15288. [pdf]
  • Tang M, Wang M, Shi C, Iglesias PA and Devreotes PN†, Huang CH†. “Evolutionarily conserved coupling of adaptive and excitable networks mediates eukaryotic chemotaxis.” Nat Commun. 2014; 5: 5175. [pdf]
  • Huang CH*, Tang M*, Shi C, Iglesias PA and Devreotes PN. “An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration.” Nat Cell Biol. 2013; 15(11): 1307-1316. [pdf]
  • Shi C, Huang CH, Devreotes PN and Iglesias PA. “Interaction of motility, directional sensing, and polarity modules recreates the behaviors of chemotaxing cells.” PLoS Comput Biol. 2013; 9(7): e1003122. [pdf]
  • Cai H, Huang CH, Devreotes PN and Iijima M. “Analysis of chemotaxis in Dictyostelium.” Methods Mol Biol. 2012; 757: 451-468. [pdf]
  • Gabelli SB, Huang CH, Mandelker D, Schmidt-Kittler O, Vogelstein B and Amzel LM. “Structural Effects of Oncogenic PI3Kα Mutations.” Curr Top Microbiol Immunol. 2011; 347: 43-53. [pdf]
  • Xiong Y, Huang CH, Iglesias PA and Devreotes PN. “Cells navigate with a local-excitation, global-inhibition-biased excitable network.” Proc Natl Acad Sci U S A. 2010; 107(40): 17079-17086. [pdf]
  • Swaney KF*, Huang CH* and Devreotes PN. “Eukaryotic chemotaxis: A network of signaling pathways controls motility, directional sensing, and polarity.” Annu Rev Biophys. 2010; 39: 265-289. [pdf]
  • Huang CH, Gabelli SB, Oldfield E and Amzel LM. “Binding of nitrogen-containing bisphosphonates (N-BPs) to the Trypanosoma cruzi farnesyl diphosphate synthase homodimer.” Proteins. 2010; 78(4): 888-899. [pdf]
  • Mandelker D, Gabelli SB, Schmidt-Kittler O, Zhu J, Cheong I, Huang CH, Kinzler KW, Vogelstein B and Amzel LM. “A frequent kinase domain mutation that changes the interaction between PI3Kα and the membrane.” Proc Natl Acad Sci U S A. 2009; 106(40): 16996-7001. [pdf]
  • Amzel LM*, Huang CH*, Mandelker D, Lengauer C, Gabelli SB, Vogelstein B. “Structural comparisons of class I phosphoinositide 3-kinases.” Nat Rev Cancer. 2008; 8: 665-669. [pdf]
  • Huang CH, Mandelker D, Gabelli SB, Amzel LM. “Insights into the oncogenic effects of PIK3CA mutations from the structure of p110α /p85α.” Cell Cycle. 2008; 7(9): 1151-6. [pdf]
  • Huang CH, Mandelker D, Schmidt-Kittler O, Samuels Y, Velculescu VE, Kinzler KW, Vogelstein B, Gabelli SB, Amzel LM. “The structure of a human p110α/p85α complex elucidates the effects of oncogenic PI3Kα mutations.” Science. 2007; 318(5857): 1744-8. [pdf]
  • Huang CH*, Peng S*, He L, Tsai YC, Boyd DA, Hansen TH, Wu TC, Hung CF. “Cancer immunotherapy using a DNA vaccine encoding a single-chain trimer of MHC class I linked to an HPV-16 E6 immunodominant CTL epitope.” Gene Ther. 2005; 12(15): 1180-6. [pdf]