Nat Methods. 2016 Aug;13(8):657-60. doi: 10.1038/nmeth.3895. Epub 2016 Jun 6.
Dense transcript profiling in single cells by image correlation decoding.
Coskun AF1,2, Cai L1,2.
Author information
Abstract
Sequential barcoded fluorescent in situ hybridization (seqFISH) allows large numbers of molecular species to be accurately detected in single cells, but multiplexing is limited by the density of barcoded objects. We present correlation FISH (corrFISH), a method to resolve dense temporal barcodes in sequential hybridization experiments. Using corrFISH, we quantified highly expressed ribosomal protein genes in single cultured cells and mouse thymus sections, revealing cell-type-specific gene expression.
PMID: 27271198 PMCID: PMC4965285 [Available on 2017-02-01] DOI: 10.1038/nmeth.3895