Core C

The acquisition and preservation of well-annotated, high-quality human tissues and biologic fluids, and the in situ analysis of the immune response therein, is pivotal to the success of all four HPV vaccine-based SPORE projects. This Shared Resource aims to support all four HPV vaccine-based SPORE projects as well as external collaborators.

We have built upon our successes in the prior funding period by expanding the resources of the Core C Shared Resource to strengthen guidance for pathologic interpretation and tissue-based analyses of immune responses, including specimens obtained from patients enrolled in SPORE-related protocols through recruitment of new members of this Shared Resource, serves as Director. This core will work closely with the Biostatistics/Bioinformatics Core (Core B) to ensure appropriate data management and controls via our web-based database as well as, study design, assay validation, and the processing and analysis of immunologic data sets.

1. Collect, process, store, and ship tissues and biologic fluids for translational research without compromising sample integrity, traceability, or patient care
2. Provide well-characterized tumors, precursor lesions and normal tissues with respect to site of origin, World Health Organization histologic subtype, differentiation, and fraction of neoplastic and stromal tissues with matching PBMC or serum samples as required
3. Support the generation of tissue microarrays with a wide range of biologic potential (e.g. normal, dysplasia, carcinoma) and/or relevant clinical parameters (e.g. before and after vaccination) and laser-capture microdissection as needed
4. Support HPV typing of samples
5. Provide well-defined mechanisms for prioritization of the expeditious distribution of requested resources to investigators within and external to the Cervical Cancer SPORE per institutional guidelines.
6. Provide expert advice, execution and interpretation of multi-parameter immunohistochemical studies including digital microscopic photography and image processing
7. Provide pathological interpretation of stained tissue and cytologic samples
8. Provide expert support and sample organization for the execution and interpretation of human serologic assays
9. Provide expert experimental support, sequencing and interpretation of human TCR sequences in tissue and blood
10. Organize blinded sample sets to ship to sites that include appropriate controls to ensure data integrity, reproducibility and comparability.

Investigators

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