Core C

The acquisition and preservation of well-annotated, high-quality human tissues and biologic fluids, and the in situ analysis of the immune response therein, is pivotal to the success of all four HPV vaccine-based SPORE projects. This Shared Resource aims to support all four HPV vaccine-based SPORE projects, current and upcoming Developmental Research Projects and Career Development Awardees as well as external collaborators. Specifically, Tissue/Pathology/Immunology Core (Core C) aims to:  

1. Collect, process, store, and ship tissues and biologic fluids for translational research without compromising sample integrity, traceability, or patient care  

2. Provide well-characterized tumors, precursor lesions and normal tissues with respect to site of origin, World Health Organization histologic subtype, differentiation, and fraction of neoplastic and stromal tissues with matching PBMC or serum samples as required  

3. Support the generation of tissue microarrays with a wide range of biologic potential (e.g. normal, dysplasia, carcinoma) and/or relevant clinical parameters (e.g. before and after vaccination) and laser-capture microdissection as needed  

4. Support HPV typing of samples 

5. Provide well-defined mechanisms for prioritization of the expeditious distribution of requested resources to investigators within and external to the Cervical Cancer SPORE per institutional guidelines.  

6. Provide expert advice, execution and interpretation of multi-parameter immunohistochemical studies including digital microscopic photography and image processing  

7. Provide pathological interpretation of stained tissue and cytologic samples  

8. Provide expert support and sample organization for the execution and interpretation of human serologic assays  

9. Provide expert experimental support, sequencing and interpretation of human TCR sequences in tissue and blood  

10. Organize blinded sample sets to ship to sites that include appropriate controls to ensure data integrity, reproducibility and comparability.  

Investigators