Models, Methods, Techniques
- Models and platforms
- Experimental animal models
– models of diffuse and focal TBI
– models of degenerative tauopathy, genetic and induced
- Experimental animal models
- Primary neuronal cultures and genetic manipulationsHumanized models of disease using human embryonic stem cell and induced pluripotent stem cell-derived precursors
- Neuropathology of the human brain
- Anatomical Methodologies
- Optimization of histology for high-resolution anatomical and in situ molecular studies
- Neuronal tract tracing and lineage tracing, injection of gene-editing CRISPR and silencing or overexpression vectors
- Immunocytochemistry/immunohistochemistry
- In situ hybridization
- High-resolution confocal and two-photon microscopy
- CLARITY and other clearing methods for high-resolution structural work on axons
- Stereology
- Electron microscopy and Focused Ion Beam Scanning Electron Microscopy
- Brainbow labeling of central neurons and their processes
- Molecular and Biochemical Methodologies
- CRISPR-, cre-lox- and shRNA-based gene editing
- Bulk, Single-cell and Spatial transcriptomics
- Ribotag-based translatomics
- Real-time PCR
- Metabolomics
- Proteomics
- Physiological methodologies
- ERG and pattern visual evoked potentials
- Neuromodulation
- Total and selective vagus nerve stimulation
- Behavioral testing