Models, Methods, Techniques

  • Models and platforms
    • Experimental animal models
      – models of diffuse and focal TBI
      – models of degenerative tauopathy, genetic and induced
    • Primary neuronal cultures and genetic manipulationsHumanized models of disease using human embryonic stem cell and induced pluripotent stem cell-derived precursors 
    • Neuropathology of the human brain
  • Anatomical Methodologies
    • Optimization of histology for high-resolution anatomical and in situ molecular studies
    • Neuronal tract tracing and lineage tracing, injection of gene-editing CRISPR and silencing or overexpression vectors
    • Immunocytochemistry/immunohistochemistry
    • In situ hybridization
    • High-resolution confocal and two-photon microscopy
    • CLARITY and other clearing methods for high-resolution structural work on axons
    • Stereology
    • Electron microscopy and Focused Ion Beam Scanning Electron Microscopy
    • Brainbow labeling of central neurons and their processes
  • Molecular and Biochemical Methodologies
    • CRISPR-, cre-lox- and shRNA-based gene editing
    • Bulk, Single-cell and Spatial transcriptomics
    • Ribotag-based translatomics
    • Real-time PCR
    • Metabolomics
    • Proteomics
  • Physiological methodologies
    • ERG and pattern visual evoked potentials
  • Neuromodulation
    • Total and selective vagus nerve stimulation
  • Behavioral testing