Techniques Employed in the Lab

  • Experimental animal models
    • models of diffuse TBI and blast TBI
    • models of degenerative tauopathy, genetic and induced
  • Optimization of histology for molecular or anatomical studies
  • Neuronal tract tracing and lineage tracing, injection of gene-editing vectors
  • Immunocytochemistry/immunohistochemistry
  • In situ hybridization
  • High-resolution fluorescence microscopy (stat-of-the art confocal, two-photon microscopy) for qualitative and quantitative studies of axons
  • CLARITY and other clearing methods for high-resolution structural work on axons
  • Electron microscopy
  • Stereology
  • Proteomics
  • CRISPR
  • Real-time PCR
  • Human embryonic stem cell and induced pluripotent stem cell manipulation and differentiation for humanized models of disease
  • Primary neuronal cultures and genetic manipulations
  • Neuropathology of the human brain