Research Interests: Mechanisms of immune surveillance and immunotherapy; Molecular mechanisms of antigen processing and presentation
Dr. Nilabh Shastri is a Bloomberg Distinguished Professor in the department of Pathology at the Johns Hopkins University School of Medicine and in the department of Biology at the Johns Hopkins University. He researches the production of molecules that serve as flags on infected or cancer cells for recognition by killer T cells. This pathway is called the antigen presentation pathway - it produces protein fragments or peptides that are chaperoned to the cell surface by MHC molecules. Here, the peptide-MHC molecules attract the attention of appropriate killer T cells which can then eliminate the flagged cells to prevent the spread of infection or cancer. Thus, the production of peptide-MHC molecules by the antigen processing pathways and the activation of appropriate T cells is the key to nearly all aspects of immunity towards microbial infections, cancer and even self-tissues in autoimmune diseases.
Shastri and his colleagues have made ground-breaking discoveries in the analysis of key steps of the antigen processing pathway. They discovered unexpected sources, such as alternate translational reading frames that are used to produce antigenic peptides for presentation by MHC molecules. Also, their discovery of peptide trimming enzymes have provided new targets for manipulating the immune response in cancer and autoimmunity.
How does the immune system detect and eliminate undesirable pathogens and regrettably, desirable transplants or self-tissues in autoimmunity.
How does our immune system know if a virus is lurking within an infected cell, or if a normal cell has turned cancerous or if an organ is transplanted from an unrelated donor? All these cells differ from our normal self, but the differences are often hidden deep within the cells’ DNA. To permit the immune system to detect these differences, our cells have evolved a remarkable mechanism, called the antigen processing and presentation pathway, that reveals intracellular contents on the surface. This otherwise hidden information is then used to elicit immunity to viruses or cancer. Virtually all intracellular proteins are fragmented into small peptides (p) that are chaperoned by MHC molecules to the cell surface (as ‘pMHC’, see figure). This comprehensive pMHC display includes peptides derived from normal as well as new proteins, for example, a viral protein in an infected cell or a mutant protein in a cancer cell. The killer T cells of the immune system use their antigen receptors to recognize specific pMHC on the surface of target cells. The pMHC thus acts as a flag for activating killer T cells which eventually eliminate the undesirable ‘foreign’ cell. Unfortunately, the same mechanisms can also reject transplanted organs and self-tissues in autoimmunity. The unique antigenic peptides that set off the killer T cells and the mechanisms that generate the pMHC in health and disease are poorly understood.
To identify unknown T cell activating peptides, we developed the ‘lacZ’ assay for measuring pMHC-specific T cell activation. T-cells containing the inducible β-galactosidase (lacZ) reporter gene are exquisite probes for detecting even rare pMHC expressing cells. Because the pMHC are generated when the antigenic precursor protein is produced in cells, we can screen DNA libraries with the lacZ T cell assay to isolate the relevant antigen coding gene. This expression cloning method has revealed the identity of many antigens from pathogenic microbes, parasites, transplanted tissues and cancer cells.
Intriguingly, we also discovered that antigenic peptides are encoded not only in conventional ‘open’ but also in alternate ‘non-coding’ cryptic translational reading frames. Such cryptic peptides are generated by a novel translation initiation mechanism and play key roles in immunity as well as normal cell biology such as the integrated stress response. We are currently studying the novel sources and translation initiation mechanisms that generate these unique peptides and their unique immunological and biological functions.
We analyze the pMHC presentation pathway using cellular, genetic and biochemical methods for tracking naturally processed peptides in antigen presenting cells. By manipulating the expression of the antigen, molecular chaperones and proteases we have defined novel steps in the pMHC antigen presentation pathway. Remarkably, manipulating the intermediate antigen processing steps inhibit or enhance immunogenicity suggesting new approaches to vaccine design, microbial immunity, metabolic disease and autoimmunity.
We are currently studying how manipulations of the antigen presentation pathway impact immune responses towards viruses, microbes, cancer and self-tissues.
Translation from the 5’ untranslated region shapes the integrated stress response. [Starck, S.R.*, J.C. Tsai, K. Chen, M. Shodiya, L. Wang, K. Yahiro, M. Martins-Green, N. Shastri*, P. Walter* (2016) Science 351: aad3861 (*Corresponding authors)]
Leucine-tRNA decodes CUG start codons for synthesis of proteins and peptides presented by MHC class I molecules. [Starck, S.R., V. Jiang, M. Pavon-Eternod, S. Prasad, B. McCarthy, T. Pan and N. Shastri(2012). Science 336:1719]
Non-classical MHC class Ib-restricted cytotoxic T cells monitor antigen processing in the endoplasmic reticulum. [Nagarajan, N., F. Gonzalez and N. Shastri (2012). Nature Immunol. 13:579-586]
The synthesis of truncated polypeptides for immune surveillance and viral evasion. [Cardinaud, S., S.R. Starck, P. Chandra and N. Shastri (2010). PLoS ONE 5:e8692]
Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum. [Blanchard, N., F. Gonzales, M. M. Schaeffer, N. T. Joncker, T. Cheng, A. Shastri, E. A. Robey, N. Shastri (2008). Nature Immunology 9:937-944]
In the absence of aminopeptidase ERAAP, MHC class I molecules present many unstable and highly immunogenic peptides. [G.E. Hammer, F. Gonzalez, E. James, H. Nolla and N. Shastri (2007) Nature Immunology 8:101-108]
Hsp90α chaperones large C-terminally extended proteolytic intermediates in the MHC I antigen processing pathway. [Kunisawa, J. and N. Shastri (2006) Immunity 24:523-534]
MHC I molecules constitutively present cryptic translation products. [S. R. Schwab, K. Li, C. Kang and N. Shastri (2003) Science 301:1367-1371]
ERAAP customizes peptides for presentation by MHC class I molecules in the endoplasmic reticulum. [T. Serwold, F. Gonzalez, J. Kim, R. Jacob and N. Shastri (2002) Nature 419, 480-483]